Product

Products and ServicesHome > Products and Services > Microbial gene knockout based on Rec homologous recombination method

  • Products
  • Performance
  • Applications
Service Introduction:
  The traditional method of bacterial gene knockout is to use the bacteria's own Rec system to perform homologous recombination of the incoming foreign DNA and its own target gene, and perform allelic replacement to achieve gene knockout. By designing a homologous fusion fragment of the target gene, it is transformed together with a suicide vector containing a recombinase, and the fragment is recombined and inserted into the target bacterial genome. Through antibiotic screening, mutant strains with integrated target sites in the bacterial genome were obtained. Under the pressure of the second round of reverse selection, only bacteria that have undergone a second homologous recombination in the bacterial genome and lost the suicide plasmid can survive. After that, the mutants with target gene deletion can be obtained by PCR identification. Gene mutants are powerful tools for studying microbial gene functions and have important scientific research value.

Tel: 17727140658 Email:shichang@tbhealthcare.com Address: Sino-European Center A, 2 Lingnan Avenue South, Shunde, Foshan, Guangdong, China
Bio-Technology Co., Ltd. Guangzhou Bo Chong Copyright Guangdong CIP prepared No. 19137094 You are the first 28835 visitors   
Services Online Services Online
Services Online Services Online